Bone Marrow Biopsy and Aspiration
Bone marrow biopsy and aspiration are procedures that collect samples of bone marrow. They are usually done together. They can help diagnose cancer and other bone marrow disorders or check how well treatment for these conditions is working.
Understanding bone marrow biopsy and aspiration
Bone marrow is the soft, spongy tissue inside certain bones where blood cells are made.
- Bone marrow aspiration involves removing a sample of the liquid part of the bone marrow.
- Bone marrow biopsy involves removing a sample of the solid part of the bone marrow.
These procedures are usually done together, but in some cases, only one might be needed. Bone marrow aspiration, which uses a smaller needle, is done first, followed by the biopsy, using a larger needle.
Most often, the aspiration and biopsy are done on the hip bone (the iliac crest). Rarely, the breastbone might be used in adults who only need bone marrow aspiration.
Why might bone marrow biopsy and aspiration be done?
Bone marrow biopsy and aspiration procedures might be done for a few reasons:
Bone marrow biopsy and aspiration procedures are often done to help diagnose a disease affecting the bone marrow or blood cells. They might be the next step after a physical exam or blood test shows something abnormal.
The samples taken are sent to the lab for testing, where they are looked at under a microscope and checked for different cell types, counts, and changes that could show disease.
Diagnosing cancers
Bone marrow biopsy and aspiration procedures are often used to diagnose cancers and cancer-related diseases. These include:
- Leukemia
- Multiple Myeloma
- Lymphoma
- Myelodysplastic Syndromes (MDS)
- Myeloproliferative neoplasms (MPNs)
- Waldenstrom Macroglobulinemia
- Some solid tumors, such as neuroblastoma or Ewing sarcoma
They can also be used once a cancer is diagnosed to see whether the tumor has spread to the bone marrow.
Diagnosing other diseases
These procedures can also help in diagnosing other diseases affecting the blood and bone marrow, including:
- Bone marrow failure (aplastic anemia)
- Other types of anemia (low red blood cells)
- Abnormal blood cell counts
Bone marrow biopsy and aspiration can also be used at certain times when someone is undergoing treatment to find out how well the treatment is working or to check if the disease has gotten worse (progressed).
Is a bone marrow biopsy painful?
Bone marrow biopsy and aspiration procedures can be painful, but the level of pain is different for each person. Some people rate the overall pain as mild to moderate, while others report it being moderate or severe.
- For a bone marrow aspiration, many people describe the pain when the liquid sample is being removed as sharp, but short-lived.
- Pain during a bone marrow biopsy is often duller and occurs when the sample of bone is removed.
Some people also have anxiety before the procedure. Because of this, some providers may prescribe medication before the procedure (premedication) to help with pain or anxiety.
Other medications may also be used to numb the area or help keep you calm. This includes different types of sedation or anesthesia:
- Local anesthesia, such as lidocaine, is often used to numb the skin over the bone marrow biopsy or aspiration site. It treats the pain but does not make you sleepy or more relaxed.
- Minimal sedation, where you are awake, alert, and able to respond but feel more relaxed.
- Conscious sedation, where you feel very drowsy, might fall asleep, and may not remember the procedure afterward.
- Deep sedation with general anesthesia where you are in a deep sleep. You might get oxygen or need a breathing tube, and an anesthesia specialist will closely monitor you the entire time. This is usually not needed for most people.
Other things that can help include:
- Using distraction and relaxation techniques during the procedure.
- Having a caregiver or a member of the cancer care team at the bedside to offer supportive comfort.
What’s it like to have a bone marrow biopsy and aspiration?
This is a general outline of what typically happens before, during, and after a bone marrow biopsy and aspiration. But your experience might be a little different, depending on why you’re having the test, where you’re having the test done, and your overall health.
Be sure to talk to your doctor before having this test so you understand what to expect. Ask questions if there’s anything you’re not sure about.
Before the procedure
You might be asked to stop taking blood-thinning medicines, including aspirin, for several days before the test to reduce the risk of bleeding.
You might be able to eat and drink like normal. Your doctor or nurse will give you specific instructions. Be sure to follow them and to ask questions if there’s anything you don’t understand.
Making a transportation plan
Depending on whether you are receiving sedation or anesthesia, you may need to arrange for a ride home after the test. If you are sleepy or dizzy, you might need someone to help you get safely into your home, so a cab or rideshare service might not be a good option. Some clinics will not allow you to use a cab or rideshare after this procedure.
If transportation or help getting home might be a problem, talk with your health care provider. There might be other resources available, depending on the situation.
At the clinic
The bone marrow biopsy and aspiration procedures are most often done in the doctor’s office or clinic, sometimes in special areas for procedures.
Be sure your health care provider knows about any medicines you are taking, including vitamins, herbs, and supplements, as well as if you have allergies to any medicines.
Once you are in the room where the procedure will be done:
- Your vital signs (temperature, blood pressure, heart rate) will be checked.
- If needed, medicines may be given to help you relax or not feel pain.
- You will be positioned on your stomach, back, or side, depending on the biopsy and aspiration site.
During the procedure
The bone marrow biopsy and aspiration procedures take about 10 to 30 minutes, depending on the type of anesthesia used. If you are getting IV sedation, extra time will be needed.
Most often, bone marrow biopsy and aspiration are done as an outpatient procedure, where you don’t need to stay overnight in a hospital. Your provider will review the procedure with you and ask you to sign a consent form.
Bone marrow aspiration
The skin over the hip area is cleaned with an antiseptic, and the area and the surface of the bone are numbed with a local anesthetic. This can cause a brief stinging or burning feeling.
Your health care provider will make a small cut in the skin and insert a thin, hollow needle through it into the bone. A syringe attached to the needle is used to suck out a small amount of liquid bone marrow. This may cause a brief, shooting pain at the area and leg, even with anesthetic. Multiple collections may be needed based on the testing to be done. Once the sample is collected, the needle is removed.
If a biopsy is not to be performed, pressure will be put on the site over the area to help stop bleeding.The samples will be sent to the laboratory for testing.
Bone marrow biopsy
Following bone marrow aspiration (if performed), a slightly larger, hollow needle is inserted through the skin into the bone. This can cause feelings of pressure as the needle is put into the proper location.
A small piece of bone and spongy marrow is removed. This can cause brief pain at the area. Once the sample is collected, the needle is removed. Pressure will be put on the site over the area to help stop bleeding.
The samples will be sent to the laboratory for testing.
After the procedure
A dressing will be placed over the biopsy area to hold pressure. You will need to stay lying down for at least 10 to 15 minutes. The dressing will need to be kept dry and in place for 24 hours.
If local anesthesia was given, usual daily activities can be restarted. If IV sedation is given, more time is needed to recover from feeling drowsy.
The biopsy area might be sore for about a week, and there might be a bruise.
Possible risks of bone marrow biopsy and aspiration
Bone marrow biopsy and aspiration are usually safe, but there is a small risk of certain complications.
Bleeding: A small amount of bleeding could happen where the provider removed the bone marrow samples. This is more likely if your platelet counts are low.
Infection: In rare cases, the cuts can get infected.
Needle breakage: Rarely, one of the needles used for bone marrow biopsy and aspiration could break during the procedure. If this happens, steps will be taken to remove it.
Drug reactions: Sometimes the sedation or anesthesia can cause changes in blood pressure, confusion, dizziness, or even an allergic reaction. You will be watched closely for any side effects, and they will be treated if needed.
Before you go home, your doctor or nurse should give you specific instructions on when you might need to call the doctor’s office for problems. In general, these include:
- Bleeding or oozing from the site that won’t stop
- Severe pain at the site
- Fever
- Redness or swelling at the site
Be sure you understand what you should watch out for, the possible timing of when problems might occur, and when you should call for problems.
- Written by
- References
The American Cancer Society medical and editorial content team
Our team is made up of doctors and oncology certified nurses with deep knowledge of cancer care as well as editors and translators with extensive experience in medical writing.
Tomasian A, Jennings JW. Bone marrow aspiration and biopsy: Techniques and practice implications. Skeletal Radiology. 2022 Jan;51(1):81-8. https://link.springer.com/article/10.1007/s00256-021-03882-w
Li C, Zhang X, Zhuang X, et al. The effectiveness of non‐pharmacological interventions on reducing pain in patients undergoing bone marrow aspiration and biopsy: A systematic review and meta‐analysis of randomized controlled trials. Journal of Clinical Nursing. 2024 Mar 28. https://doi.org/10.1111/jocn.17121
Srivastava U, Pezeshk P, Chhabra A. Patient satisfaction experience and outcomes after CT-Guided bone marrow biopsy versus in-office bone marrow biopsy. Radiation. 2024 Aug 2;4(3):224-31. https://doi.org/10.3390/radiation4030017
Zehnder JL. Bone marrow aspiration and biopsy: Indications and technique. UpToDate. 2026. Accessed at https://www.uptodate.com/contents/bone-marrow-aspiration-and-biopsy-indications-and-technique on March 12, 2026
Last Revised: March 26, 2026
American Cancer Society medical information is copyrighted material. For reprint requests, please see our Content Usage Policy.
American Cancer Society Emails
Sign up to stay up-to-date with news, valuable information, and ways to get involved with the American Cancer Society.